Sialic Acid Analysis by HPAEC-PAD
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The most labile monosaccharides are the family of sialic acids, which are usually chain-terminating substituents. These are therefore usually released first by either mild acid hydrolysis or enzyme digestion, and can be analyzed with great sensitivity by high pH anion-exchange chromatography with pulsed auperometric detection (HPAEC-PAD). The remaining oligosaccharide is analyzed as discussed in Chapter 103 to identify the position of linkage of the sialic acid.
KeywordsSialic Acid Newcastle Disease Virus Newcastle Disease Chromic Acid Fucose Residue