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Sialic Acid Analysis by HPAEC-PAD

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith
Protocol
  • 72 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The most labile monosaccharides are the family of sialic acids, which are usually chain-terminating substituents. These are therefore usually released first by either mild acid hydrolysis or enzyme digestion, and can be analyzed with great sensitivity by high pH anion-exchange chromatography with pulsed auperometric detection (HPAEC-PAD). The remaining oligosaccharide is analyzed as discussed in Chapter 103 to identify the position of linkage of the sialic acid.

Keywords

Sialic Acid Newcastle Disease Virus Newcastle Disease Chromic Acid Fucose Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Davies, M. J., Smith, K. D., and Hounsell, E. F. (1994) The release of oligosaccharides from glycoproteins, in Methods in Molecular Biology, vol. 32: Basic Protein and Peptide Protocols (Walker, J. M., ed.), Humana, Totowa, NJ, pp. 129–141.CrossRefGoogle Scholar
  2. 2.
    Townsend, R. R. (1995) Analysis of glycoconjugates using high-pH anion-exchange chromatography. J. Chromatog. Library 58, 181–209.CrossRefGoogle Scholar
  3. 3.
    Manzi, A. E., Diaz, S., and Varki, A. (1990) HPLC of sialic acids on a pellicular resin anion exchange column with pulsed amperometry. Anal. Biochem. 188, 20–32.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
    • 1
  • Kevin D. Smith
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyUniversity College LondonUK
  2. 2.Department of Pharmaceutical SciencesUniversity of StrathclydeGlasgowScotland, UK

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