Rodent Model of Pediatric Asphyxial Cardiac Arrest

  • Mioara D. Manole
  • Robert W. Hickey
  • Henry L. Alexander
  • Robert S.B. Clark
Part of the Springer Protocols Handbooks book series (SPH)

A model of asphyxial cardiac arrest in 17-day old rats is described. This clinically relevant model includes a period of hypoxemia followed by ischemia and resuscitation. Continuous physiologic monitoring is performed before, during, and after the insult. Graded insults produce consistent and dose-dependent brain injury with histological damage and behavioral impairment. The details of the procedures, along with outcome assessments and applications, are discussed.


Asphyxia Cardiac arrest Cerebral blood flow Neurodegeneration Sprague-Dawley rat 


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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Mioara D. Manole
    • 1
  • Robert W. Hickey
    • 1
  • Henry L. Alexander
    • 1
  • Robert S.B. Clark
    • 1
  1. 1.Department of Pediatrics, Division of Pediatric Emergency MedicineSafar Center for Resuscitation Research, Children's Hospital of PittsburghPittsburghUSA

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