Rodent Spinal Cord Demyelination Models

  • Sarah C. Jernigan
  • Yi Ping Zhang
  • Christopher B. Shields
  • Scott R. Whittemore
Part of the Springer Protocols Handbooks book series (SPH)

Demyelination is a significant component of contusive spinal cord injury as well as in multiple sclerosis. As such, remyelination, either by endogenous or grafted exogenous myelinating cells is a viable therapeutic target to restore function. To assess specific approaches to facilitate functional remyelination in vivo, appropriate injury models are needed. This chapter will discuss the strengths and weaknesses of a number of demyelinating lesions of the spinal cord and provide guidelines for choosing which model best suits which experimental condition. Step by step procedures for both creating and assessing the lesion will be provided.


Spinal cord injury Demyelination Ethidium bromide Lysolethicin Cuprizone 


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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Sarah C. Jernigan
    • 1
  • Yi Ping Zhang
    • 1
  • Christopher B. Shields
    • 1
  • Scott R. Whittemore
    • 1
  1. 1.Department of Neurological Surgery, Kentucky Spinal Cord Injury Research CenterUniversity of Louisville School of MedicineLouisvilleUSA

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