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Cerebral Hypoxia—Ischemia Model in Adult Mice

  • Faisal Adhami
  • Chia-Yi Kuan
Protocol
  • 2k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Cerebral hypoxia—ischemia in adult mice allows the study of the effects of hypoxia in a partially ischemic territory. This model may be clinically relevant in situations of general respiratory-circulatory failure (e.g., in shock) or ischemic tissue that displays a breakdown in oxygen metabolism during stroke progression. In this chapter, we outline this procedure in detail from ligation of the common carotid to administration of the hypoxic gas. We will also describe outcome measurements using behavioral deficits, Nissl stain infarct quantification, and fluorescein isothiocyanate-dextran (FITC-dextran) perfusion marking. Finally, we will briefly discuss the advantages, limitations, and complications with using cerebral hypoxia-ischemia in adult rodents.

Keywords

Ischemia Hypoxia No-reflow Reperfusion Thrombosis Autophagy 

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Faisal Adhami
    • 1
  • Chia-Yi Kuan
    • 1
  1. 1.Department of Pediatrics, Division of Developmental Biology and NeurologyChildren's Hospital Medical CenterCincinnatiUSA

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