Summary
Atomic force microscopy (AFM) measurements of intermolecular binding strength between a single pair of complementary cell adhesion molecules in physiological solutions provided the first quantitative evidence for their cohesive function. This novel AFM-based nanobiotechnology opens a molecular mechanic approach for studying structure- to function-related properties of any type of individual biological macromolecules. The presented example of Porifera cell adhesion glyconectin proteoglycans showed that homotypic carbohydrate to carbohydrate interactions between two primordial proteoglycans can hold the weight of 1,600 cells. Thus, glyconectin type carbohydrates, as the most peripheral cell surface molecules of sponges (today’s simplest living Metazoa), are proposed to be the primary cell adhesive molecules essential for the evolution of the multicellularity.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dammer, U., Popescu, O., Wagner, P., Anselrnetti, D., Guntherodt, H. I., and Misevic, G. N.(1995)Binding strength between cell adhesion proteoglycans measured by atomic force microscopy. Science267, 1173–1175.
Binnig, G., Quare, C. F., and Gerber, C.(1986)Atomic force microscopy. Phys. Rev. Lett.56, 930.
Hermanson, G. T., Mallia, K. A., and Smith, P. K.(1992)Immobilised Affinity Techniques. Academic, New York.
Bucior, I., Scheuring, S., Engel, A., Burger, M. M.(1994)Carbohydrate–carbohydrate interaction provides adhesion force and specificity for cellular recognition. J Cell Biol.165, 529–537.
Lee, G. U., Kidwell, D. A., and Colton, R. C.(1994)Sensing discrete streptavidin–biotin interactions with atomic force microscopy. Langmuir10, 354–357.
Florin, E. L., Moy, V. T., and Gaub, H. E.(1994)Adhesion forces between individual ligand–receptor pairs. Science264, 415–417.
Acknowledgments
This work was supported by the private funds of Dr. G. N. Misevic, Conseil Regional Nord-Pas de Calais, CNRS, Marcel Merieux Foundation, Swiss National Science Foundation, Cancer League Basel, and sixth framework programme Network of Excellence Nanobeams.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Misevic, G., Karamanos, Y., Misevic, N. (2009). Atomic Force Microscopy Measurements of Intermolecular Binding Forces. In: Even-Ram, S., Artym, V. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology, vol 522. Humana Press. https://doi.org/10.1007/978-1-59745-413-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-59745-413-1_8
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-58829-984-0
Online ISBN: 978-1-59745-413-1
eBook Packages: Springer Protocols