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Flow Cytometry

  • Robert E. Cunningham
Protocol
  • 1k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Flow cylometry (FCM) is a powerful technique for rapid analysis and sorting (separation) of cells and particles In theory, it can be used to measure any cell component, provided that a measurable physical property or fluorescent tracer is available that reacts specifically and stoichiometrically with that constituent, i e , cell-surface receptor, DNA, intracellular receptor, RNA, or chromosomes The technique provides statistical accuracy, reproducibihty, and sensitivity. For approximately the past 20 years FCM has enjoyed increasing utilization in the areas of hematology, immunology, cancer biology, pharmacology and karyotype analysis, mechanisms of multidrug resistance (MDR), cell-cycle analysis, chromosome-ploidy analysis, and analysis for somatic-cell genetics and chromosome sorting (1)

Keywords

Scattered Laser Light Fluorescent Tracer Chromosome Sorting Result Flow Cytometer Rare Cell Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Further Reading

  1. Flow Cytometry and Sortzng, Myron R Melamed, Paul F Mullaney, Mortlmer L Mendelsohn (eds) New York Wiley, 1979, 716 pGoogle Scholar
  2. Flow Cytometry and Sortrng, Myron R Melamed, Tore Lmdmo, Mortlmer L Mendelsohn (eds), New York Wiley-Liss, 1990, 824 pGoogle Scholar
  3. Practical Flow Cytometry, Howard M Shapiro New York Llss, 1985, 295 pGoogle Scholar
  4. Practical Flow Cytometry, Howard M Shapiro New York Liss, 1988, 353 pGoogle Scholar
  5. Practical Flow Cytometry, Howard M Shapiro New York Wiley-Llss, 1995, 542 pGoogle Scholar
  6. Flow Cytometry IV Proceedings of the IVth International Symposium on Flow Cytometry (Pulse Cytophotometry), O D Laerum, T Lmdmo, E Thorud (eds), New York Columbia University Press, 1980, 535 pGoogle Scholar
  7. Flow Cytometry, A Powerful Technology for Measurzng Blomarkers, James H Jett (ed), Los Alamos, Los Alamos, NM Los Alamos National Laboratory, 1994Google Scholar
  8. Flow Cytometry, Zbigniew Darzynklewicz, J Paul Robinson, Harry A Crlssman (eds) San Diego Academic Press, 1994Google Scholar
  9. Flow Cytometry, Practical Approach, M G Ormerod (ed), New York IRL Press, 1994,282 pGoogle Scholar
  10. Flow Cytometry Cllnlcal Apphcatlons, Marion G Macey (ed), Boston Blackwell Scientific Publications, 1994Google Scholar
  11. Handbook of Flow Cytometry Methods, J Paul Robinson, (ed), New York Wiley-Liss, 1993, 246 pGoogle Scholar
  12. Flow Cytometry and Clinical Diagnosis, David F Keren, Curtis A Hanson, Paul Hurtublse (eds), Chicago ASCP Press, 1993Google Scholar
  13. Flow Cytometry In Clinical Diagnosis, David F Keren (ed), Chicago ASCP Press, 1989, 34313Google Scholar
  14. Clinical Applications of Flow Cytometry, Roger S. Riley, Edwin J Mahm, Wdham Ross (eds), New York Igaku-Shom, 1993, 914 pGoogle Scholar
  15. Clinical Flow Cytometry, Alan L Landay (ed), New York New York Academy of Sciences, 1993, 468pGoogle Scholar
  16. Clinical Flow Cytometry Principles and Application, Kenneth D Bauer, Ricardo E Duque, T Vincent Shankey (eds), Baltimore Wtlhams & Wllkms, 1993, 635 pGoogle Scholar
  17. Introduction to Flow Cytometry, James V. Watson (ed), New York Cambridge University Press, 1991, 443 pGoogle Scholar
  18. Flow Cytometry and Cell Sorting, A Radbruch (ed), New York Springer-Verlag, 1992,223 pGoogle Scholar
  19. Flow Cytogenetlcs, Joe W Gray (ed), San Diego Academic Press, 1989, 312 pGoogle Scholar

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Copyright information

© Humana Press Inc , Totowa, NJ. 1998

Authors and Affiliations

  • Robert E. Cunningham
    • 1
  1. 1.Department of Cellular PathologyAFIPWashington

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