M13 and Phagemid-Based Cloning Vectors

  • Ralph Rapley
Part of the Springer Protocols Handbooks book series (SPH)


Bacteriophage-based vectors, such as λ have been useful in creating gene llbrarles and in the expression of proteins However, a number of techniques used in molecular biology require DNA to be ina single-stranded form, such as chain termmatlon sequencing, ollgonucleotlde-directed mutagenesis, and certain probe synthesis strategies A small number of naturally occurring viruses produce single-stranded DNA as part of their life cycle, these include M13, fl , and fd, and a number of them have been mampulated and adapted as cloning vectors for the purpose of single-strand DNA production


Cloning Vector lacZ Gene Replicative Form Helper Phage Phagemid Vector 
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Copyright information

© Humana Press Inc , Totowa, NJ 1998

Authors and Affiliations

  • Ralph Rapley
    • 1
  1. 1.Unzverszty of HertfordshzreUK

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