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M13 and Phagemid-Based Cloning Vectors

  • Ralph Rapley
Protocol
  • 1.2k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Bacteriophage-based vectors, such as λ have been useful in creating gene llbrarles and in the expression of proteins However, a number of techniques used in molecular biology require DNA to be ina single-stranded form, such as chain termmatlon sequencing, ollgonucleotlde-directed mutagenesis, and certain probe synthesis strategies A small number of naturally occurring viruses produce single-stranded DNA as part of their life cycle, these include M13, fl , and fd, and a number of them have been mampulated and adapted as cloning vectors for the purpose of single-strand DNA production

Keywords

Cloning Vector lacZ Gene Replicative Form Helper Phage Phagemid Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc , Totowa, NJ 1998

Authors and Affiliations

  • Ralph Rapley
    • 1
  1. 1.Unzverszty of HertfordshzreUK

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