Advertisement

Solution Properties of Proteins

  • Felix Franks
Protocol
Part of the Biological Methods book series (BM)

Abstract

Physicochemical measurements find application principally in the determination of size, charge, shape, conformation, interactions (e.g., binding, hydration), and dynamics (diffusion, reaction rates). Most techniques are limited to dilute solutions; some require optical transparency.

Keywords

Nuclear Magnetic Resonance Circular Dichroism Nuclear Magnetic Resonance Spectrum Circular Dichroism Spectrum Unpaired Electron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Jaenicke R. (1980) Protein Folding, Elsevier, Amsterdam.Google Scholar
  2. 2.
    Schellman, J. A. and Hawkes R. B. (1980) in Protein Folding (Jaenicke, R., ed.) Elsevier, Amsterdam, p. 331.Google Scholar
  3. 3.
    Tanford C. (1961) Physical Chemistry of Macromolecules, John Wiley & Sons, New York, Chap. 8.Google Scholar
  4. 4.
    Hofmeister R (1888) Arch. Exptl. Pathol. Pharmakol. 24, 247–260.CrossRefGoogle Scholar
  5. 5.
    von Hippel P. H. and Schleich T. (1969) Acc. Chem. Res. 2, 257–265.CrossRefGoogle Scholar
  6. 6.
    von Hippel P. H. and Wong K. Y. (1965) J. Biol. Chem. 240, 3909–3923.Google Scholar
  7. 7.
    Hamabata A. and von Hippel P. (1973) Biochemistry 12, 1256–1261.CrossRefGoogle Scholar
  8. 8.
    Arakawa T. and Timasheff S. N. (1982) Biochemistry 21, 6536–6544.PubMedCrossRefGoogle Scholar
  9. 9.
    Uedaira H. (1980) Bull. Chem. Soc. Japan 53, 2451–2455.CrossRefGoogle Scholar
  10. 10.
    Gekko K and Morikawa T. (1981) J. Biochem. 90, 39–50.PubMedGoogle Scholar
  11. 11.
    Franks F. and Eagland D. (1975) Crit. Rev. Biochem. 3, 165–219.CrossRefGoogle Scholar
  12. 12.
    Brandts J. F. and Hunt L. (1967) J. Amer. Chem. Soc. 89, 4826–4840.CrossRefGoogle Scholar
  13. 13.
    Franks F. and Desnoyers J. E. (1985) Water Sci. Rev. 1, 171–232.CrossRefGoogle Scholar
  14. 14.
    Richards E. G. (1980) An Introduction to Physical Chemistry of Large Mol ecules in Solution, Cambridge University Press, Cambridge, UK.Google Scholar
  15. 15.
    van Holde K. E. (1971) Physical Biochemistry, Prentice-Hall, NJ.Google Scholar
  16. 16.
    Eisenberg H. (1976) Biological Macromolecules and Polyelectrolytes in Solution, Clarendon Press, Oxford.Google Scholar
  17. 17.
    Mahler, H. R. and Cordes E. H. (1966) Biological Chemistry, Harper & Row, New York.Google Scholar
  18. 18.
    Franks F. and Morris E. R. (1978) Biophys. Biochim. Ada 540, 346–356.Google Scholar
  19. 19.
    Utsumi H., Yamazaki S., Hosoi K., Kimura S., Hanada K., Shimazu T., and Shimizu H. (1987) J. Biochem. 101, 1199–1208.PubMedGoogle Scholar
  20. 20.
    Dwek R. A. (1973) Nuclear Magnetic Resonance in Biochemistry Clarendon Press, Oxford (1973).Google Scholar
  21. 21.
    Markley J. L. (1987) in Protein Engineering (Oxender D. L. and Fox C. F., eds.), Liss, New York, pp. 15–25.Google Scholar
  22. 22.
    Franks F., Dadok J., Ying S., Kay R. L., and Grigera J. R. (1991) J. Chem. Soc. Faraday Trans. 87, 579–585.CrossRefGoogle Scholar
  23. 23.
    Forsen S., Thulin E., Drakenberg T., Krebs J., and Scamon K. (1980) FEBS Lett. 117, 189–194.PubMedCrossRefGoogle Scholar
  24. 24.
    Markley J. L., Westler W. M., Chan T.-M., Kojiro C. L., and Ulrich E. L. (1984) Fed. Proc. 43, 2648–2654.PubMedGoogle Scholar
  25. 25.
    Kaptein R., Boelens R., Scheek R. M., and van Gunsteren W. F. (1988), Biochemistry 27, 5389–5395.PubMedCrossRefGoogle Scholar
  26. 26.
    Oschkinat H., Griesinger C., Kraulis P. J., Sorensen O. W., Ernst, R. C., Gronenborn, A. M., and Clore, G. M. (1988) Nature 332, 374–376.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc 1993

Authors and Affiliations

  • Felix Franks
    • 1
  1. 1.Pafra LtdCambridgeEngland

Personalised recommendations