Analytical Chromatography of Amino Acids, Peptides, and Proteins

  • James L. Dwyer
Part of the Biological Methods book series (BM)


A broad range of separatory modes and media are utilized to identify and characterize the polypeptides and amino acids. The analytical chromatography of polypeptides also encompasses various preparative modes, as chromatography is frequently used to isolate materials for other analytical procedures. High molecular weight proteins are separated both in denatured and active configuration states. In this chapter an arbitrary distinction is made between the low molecular weight peptides (<20,000 Da) and the high molecular weight proteins. Both the kinetic and chemical behavior of the polypeptides within the chromatographic environment varies markedly as molecular weight is increased. The chapter is organized according to the principal chromatography modes, with recognition of special considerations that encompass the differences in physical/chemical behavior between small peptides and protein macromolecules.


High Performance Liquid Chromatography High Performance Liquid Chromatography Capacity Factor Solute Molecule High Molecular Weight Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Horvath, C. (1983), High Performance Liquid Chromatography, vol. 3, Academic, p. 120.Google Scholar
  2. 2.
    Affinity Chromatography Supports Narayanan, Sunanda R., Crane, Laura, and Tibtech, A., eds., vol. 8, (Jan 90), pp. 12–16.Google Scholar
  3. 3.
    Chromatographic Separations of Stereoisomers (1985), Souter, R. W., ed., CRC, Boca Raton, FL.Google Scholar
  4. 4.
    Davankov, V. A., Bochkov, A. S., Kurgahov, A. A., Roumeliotis, P., and Unger, K. K. (1980), Chromatographia 13, 677.CrossRefGoogle Scholar
  5. 5.
    Gross, D. (1955), Nature 184, 1298.CrossRefGoogle Scholar
  6. 6.
    Consdon, R., Gordon, A. H., and Martin, J. P. (1944), Biochem. J. 8, 224.Google Scholar
  7. 7.
    Dent, C. E. (1948), Biochem. J. 43, 169.Google Scholar
  8. 8.
    Spackman, D. H., Stein, W. H., and Moore, S. (1958), Anal. Chern. 31, 1190.Google Scholar
  9. 9.
    Horvath, C. High Performance Liquid Chromatography, vol. 3, Academic, p. 55.Google Scholar

Copyright information

© The Humana Press Inc 1993

Authors and Affiliations

  • James L. Dwyer
    • 1
  1. 1.Ventec Inc.Marlborough

Personalised recommendations