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Use and Production of Polyclonal and Monoclonal Antibodies

  • A. W. Schram
Protocol
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Part of the Biological Methods book series (BM)

Abstract

To obtain information on the properties and structure of proteins, the use of specific probes is of great advantage in many cases. These probes may be, for example, substrate analogs (in case of enzymes) that firmly bind to the enzyme, or even suicide substrates that bind and inactivate the enzyme. Another group of very specific probes is represented by antibodies that specifically recognize structural parts of the protein. Over the last 15 years, the use of antibodies in detecting, characterizing, and purifying proteins has become a common method.

Keywords

Spleen Cell Myeloma Cell Thymidine Kinase Antigenic Site Cloning Procedure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1988

Authors and Affiliations

  • A. W. Schram
    • 1
  1. 1.Vakgroep BiochemieUniversiteit van Amsterdam, Academisch Medisch CentrumAmsterdamThe Netherlands

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