Use and Production of Polyclonal and Monoclonal Antibodies
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To obtain information on the properties and structure of proteins, the use of specific probes is of great advantage in many cases. These probes may be, for example, substrate analogs (in case of enzymes) that firmly bind to the enzyme, or even suicide substrates that bind and inactivate the enzyme. Another group of very specific probes is represented by antibodies that specifically recognize structural parts of the protein. Over the last 15 years, the use of antibodies in detecting, characterizing, and purifying proteins has become a common method.
KeywordsSpleen Cell Myeloma Cell Thymidine Kinase Antigenic Site Cloning Procedure
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