Preparative Liquid Chromatography
- 332 Downloads
Preparative liquid chromatography (PLC) potentially can be undertaken using all of the techniques described in Chapter 11, and the basic requirement for efficient preparation is to obtain the maximum throughput in unit time. This requires optimization of the methods employed, which is the subject of this chapter. However, there is an additional constraint in preparative protein separations; that is, the prepared proteins should retain their biological activity, and this imposes severe restraints upon the operating system. Three effects can be listed that potentially can affect protein denaturization: (1) the “hardness” of the surface, (2) temperature effects, and (3) shear forces.
KeywordsMaximum Throughput Column System Solvent Consumption Preparative Column Preparative Liquid Chromatography
- 2.R. P. W. Scott in Contemporary Liquid Chromatography. John Wiley, New York (1976).Google Scholar
- 3.Hazel Pyper, Doctoral thesis, University of Edinburgh, Scotland(1984)Google Scholar
- 5.J. J. de Stefano and H. C. Beachell, J Chromatogr. Sci. 8, 434 (1970).Google Scholar
- 6.C. E. Reese, in Techniques in Liquid Chromatography (C. F. Simpson, ed ) Wiley-Heyden, John Wiley (1982).Google Scholar