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Preparative Liquid Chromatography

  • Colin Simpson
Protocol
  • 332 Downloads
Part of the Biological Methods book series (BM)

Abstruct

Preparative liquid chromatography (PLC) potentially can be undertaken using all of the techniques described in  Chapter 11, and the basic requirement for efficient preparation is to obtain the maximum throughput in unit time. This requires optimization of the methods employed, which is the subject of this chapter. However, there is an additional constraint in preparative protein separations; that is, the prepared proteins should retain their biological activity, and this imposes severe restraints upon the operating system. Three effects can be listed that potentially can affect protein denaturization: (1) the “hardness” of the surface, (2) temperature effects, and (3) shear forces.

Keywords

Maximum Throughput Column System Solvent Consumption Preparative Column Preparative Liquid Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    R. P. W. Scott and P. Kucera, J. Chromafogr. 119, 467 (1976).CrossRefGoogle Scholar
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    R. P. W. Scott in Contemporary Liquid Chromatography. John Wiley, New York (1976).Google Scholar
  3. 3.
    Hazel Pyper, Doctoral thesis, University of Edinburgh, Scotland(1984)Google Scholar
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    M._Martin, F. Verillon, C. Eon, and G. Guiochon. J. Chromafogr. 125,17 (1976).CrossRefGoogle Scholar
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    J. J. de Stefano and H. C. Beachell, J Chromatogr. Sci. 8, 434 (1970).Google Scholar
  6. 6.
    C. E. Reese, in Techniques in Liquid Chromatography (C. F. Simpson, ed ) Wiley-Heyden, John Wiley (1982).Google Scholar

Copyright information

© The Humana Press Inc 1988

Authors and Affiliations

  • Colin Simpson
    • 1
  1. 1.Department of ChemistryChelsea College,Universzty of LondonLondonUK

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