Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis
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book series (BM)
Polyacrylamide gel electrophoresis (PAGE) is one of the most widely used techniques for the analytical separation of proteins and peptides. PAGE separations, based upon intrinsic charge-to-mass ratio (1), apparent molecular weight in the presence of sodium dodecyl sulfate (SDS) (2), and isoelectric focusing (3), all possess remarkable resolving power using amounts of protein ranging from a few picograms to several milligrams. When two of these techniques, molecular sieving with SDS and isoelectric focusing with ampholytes, are combined in two-dimensional separations (4), complex mixtures can be resolved into as many as several thousand components in a single experiment.
KeywordsSodium Dodecyl Sulfate Ammonium Bicarbonate Dialysis Membrane Fraction Collector Sodium Dodecyl Sulfate Solution
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Ornstein, L (1964) Ann. NY Acad. Sci.
, 321–349.PubMedCrossRefGoogle Scholar
Laemmli, U. K. (1970) Nature (Lond)
, 680–685.CrossRefGoogle Scholar
Catsimpoolas, N. (1970) Sep. Sci.
, 523–545.CrossRefGoogle Scholar
O’Farrell, P. H. (1975) J. Biol. Chem.
, 4007–4021.PubMedGoogle Scholar
Anderson, C. W., Baum, P. R., and Gesteland, R. F. (1973) J. Virol
, 241–253.PubMedGoogle Scholar
Lazarides, E. (1976) J. Supramol. Struct.
, 531–547.PubMedCrossRefGoogle Scholar
Hunkapiller, M. W., Lujan, E., Ostrander, F., and Hood, L. E. (1983) Methods Enzymol.
, 227–236.PubMedCrossRefGoogle Scholar
Merril, C. R., Goldman, D., Sedman, S. A., and Ebert, M. H. (1981) Science
, 1437–1438.PubMedCrossRefGoogle Scholar
Wessel, D. and Flugge, U. I. (1984) Anal. Biochem
, 141–143.PubMedCrossRefGoogle Scholar
© The Humana Press Inc. 1986