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Analysis of Phenylthiohydantoin Amino Acids by HPLC

  • Michael W. Hunkapiller
  • Kristina Granlund-Moyer
  • Norman W. Whiteley
Protocol
Part of the Biological Methods book series (BM)

Abstract

High performance liquid chromatography (HPLC) on reverse-phase, silica-based supports has revolutionized protein-sequencing analytical procedures. It provides rapid, sensitive, quantitative analysis of phenylthiohydantoin (PTH) amino acids formed during the Edman degradation of proteins and peptides. It is the only technique commonly used for PTH analysis that can resolve all of the PTH amino acids in a single chromatographic run. Because it provides quantitative data at the pmol level, HPLC is also the only available method suitable for microsequencing that uses the new generation of automated Edman sequencers (1,2).

Keywords

High Performance Liquid Chromatography High Performance Liquid Chromatography High Performance Liquid Chromatography System Sequencer Fraction High Performance Liquid Chromatography Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc. 1986

Authors and Affiliations

  • Michael W. Hunkapiller
    • 1
  • Kristina Granlund-Moyer
    • 1
  • Norman W. Whiteley
    • 1
  1. 1.Applied BiosystemsFoster City

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