Analysis of Phenylthiohydantoin Amino Acids by HPLC
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High performance liquid chromatography (HPLC) on reverse-phase, silica-based supports has revolutionized protein-sequencing analytical procedures. It provides rapid, sensitive, quantitative analysis of phenylthiohydantoin (PTH) amino acids formed during the Edman degradation of proteins and peptides. It is the only technique commonly used for PTH analysis that can resolve all of the PTH amino acids in a single chromatographic run. Because it provides quantitative data at the pmol level, HPLC is also the only available method suitable for microsequencing that uses the new generation of automated Edman sequencers (1,2).
KeywordsHigh Performance Liquid Chromatography High Performance Liquid Chromatography High Performance Liquid Chromatography System Sequencer Fraction High Performance Liquid Chromatography Column
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