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Microwave Fixation of Rat Hippocampal Slices

  • Marcia D. Feinberg
  • Karen M. Szumowski
  • Kristen M. Harris
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The authors’ research focuses on the structural basis of synaptic function. Knowing the dimensions and connectivity of presynaptic and postsynaptic elements is necessary for understanding the mechanisms of synaptic transmission in the central nervous system. To this end, electrophysiological (EP) methods are used in combination with confocal microscopy, serial electron microscopy (EM), three-dimensional reconstruction, and quantitative image analysis. Microwave (MW)-enhanced methods for preparing hippocampal (HC) slices are described in this chapter. These procedures have been adapted from (1989) and (1985).

Keywords

Cacodylic Buffer Water Load Tissue Preservation Oven Cavity Artificial Cerebral Spinal Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Marcia D. Feinberg
    • 1
  • Karen M. Szumowski
    • 1
  • Kristen M. Harris
    • 1
  1. 1.Department of BiologyBoston UniversityBoston

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