Overview of Microwave-Assisted Tissue Processing for Transmission Electron Microscopy

  • Richard S. DemareeJr.
  • Richard T. Giberson
Part of the Springer Protocols Handbooks book series (SPH)


The purpose of this book is to provide new, reliable, and recently updated protocols for processing many different kinds of samples, using microwave (MW) technology. Chapters included deal with a wide variety of methods and samples, everything from paraffin processing for light microscopy (LM) to scanning and transmission electron microscopy (TEM), to immunocytochemistry.


Temp Probe Water Load Fixation Step Subsequent Chapter Resin Infiltration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Demaree RS Jr, Giberson RT, Smith RL (1995) Routine microwave polymerization of resins for transmission electron microscopy. Scanning 17(Suppl. 5):25–26.Google Scholar
  2. Fox NE, Demaree RS Jr. (1999) Quick bacterial microwave fixation technique for scanning electron microscopy. Microsc Res Tech 46:338–339.PubMedCrossRefGoogle Scholar
  3. Giammara BL (1993) Microwave embedment for light and electron microscopy using epoxy resins, LR White, and other polymers. Scanning 15:82–87.Google Scholar
  4. Giberson RT, Demaree RS Jr (1995) Microwave fixation: understanding the variables to achieve rapid reproducible results. Microsc Res Tech 32:246–254.PubMedCrossRefGoogle Scholar
  5. Giberson RT, Demaree RS Jr (1999) Microwave processing technique for electron microscopy: a four-hour protocol. In: (Hajibagheri N, ed.) Electron Microscopy Methods and Protocols. Humana, Totowa, NJ, pp. 145–158.CrossRefGoogle Scholar
  6. Giberson RT, Demaree RS Jr, Nordhausen RN (1997) Four hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J Vet Diagn Invest 9:61–67.PubMedGoogle Scholar
  7. Hayat MA (1989) Principles and Techniques of Electron Microscopy. Biological Applications, 3rd ed. CRC Press, Boca Raton, FL.Google Scholar
  8. Login GR, Dvorak AM (1982) Microwave energy fixation of tissue specimens for light and electron microscopy studies. Acad Gen Dentistry 30:92 (abstract).Google Scholar
  9. Login GR, Dvorak AM (1993) Review of rapid microwave fixation technology: its expanding niche in morphologic studies. Scanning 15:58–66.PubMedGoogle Scholar
  10. Login GR, Dvorak AM (1994) The Microwave Toolbook. A Practical Guide for Microscopists. Beth Israel Hospital, Boston, MA.Google Scholar
  11. Login GR, Tanda N, Dvorak AM (1996) Calibrating and standardizing microwave ovens for microwave-accelerated specimen preparation. Cell Vision 3:172–179.Google Scholar
  12. Mayers CP (1970) Histological fixation by microwave heating. J Clin Pathol 23:273–275.PubMedCrossRefGoogle Scholar
  13. Zimmerman GR, Raney JA (1972) Fast fixation for surgical pathology specimens. Lab Med 3:29–30.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Richard S. DemareeJr.
    • 1
  • Richard T. Giberson
    • 2
  1. 1.Department of Biological SciencesCalifornia State UniversityChico
  2. 2.Ted Pella Inc.Redding

Personalised recommendations