Mouse Models to Study Antiobesogenic Effects of Carotenoids

  • Joan RibotEmail author
  • Bojan Stojnic
  • Andreu Palou
  • M. Luisa Bonet
Part of the Methods in Molecular Biology book series (MIMB, volume 2083)


Carotenoids entail a vast potential to tackle health problems including obesity and some of its comorbidities. The use of animal models remains necessary, particularly at early stages of research (preclinical) and for advancing in mechanistic aspects of carotenoid action. No single animal model completely mimics human absorption and metabolism of carotenoids, and the best model must be chosen considering the specific application, characteristics of the individual models, and funding and facilities available. Here, we propose three protocols in mice to investigate the potential of a given carotenoid, carotenoid mixture, or carotenoid-rich extract to (a) counteract the development of obesity and prevent the metabolic alterations caused by feeding mice a moderate high-fat diet; (b) improve the metabolic profile of obese animals with metabolic alterations caused by chronic high-fat diet feeding; and (c) act as coadjuvants in weight loss strategies (reversion to a low fat diet) applied to diet-induced obese animals.

Key words

Animal models Obesity Metabolic syndrome Carotenoids 



BS is the recipient of a “La Caixa” Foundation predoctoral contract at the University of Balearic Islands. The group is a member of the European COST-Action EUROCAROTEN (CA15136; EU Framework Programme Horizon 2020), and the Spanish Network of Excellence CaRed (BIO2015-71703-REDT and BIO2017-90877-REDT; Agencia Estatal de Investigación, MICIU/FEDER, EU). CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn) is an initiative of the ISCIII (Spanish Government).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Joan Ribot
    • 1
    Email author
  • Bojan Stojnic
    • 1
  • Andreu Palou
    • 1
  • M. Luisa Bonet
    • 1
  1. 1.Grup de Recerca Nutrigenòmica i Obesitat, Laboratori de Biologia Molecular, Nutrició i Biotecnologia (LBNB) of the Universitat de les Illes BalearsCIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn) and Institut d’Investigació Sanitària Illes Balears (IdISBa)Palma de MallorcaSpain

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