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In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery

  • Diana CaridhaEmail author
  • Susan Leed
  • Alicia Cawlfield
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2081)

Abstract

Confirming the in vivo efficacy of potential antileishmanial compounds that display in vitro potency and good chemical characteristics is one of the most important steps in preclinical research drug discovery before human clinical trials begin. Here we describe the use of the in vivo bioluminescent monitoring of high and low inocula of luciferase-expressing Leishmania major (L. major) parasites in traditional and more innovative rodent models of in vivo cutaneous leishmaniasis (CL) drug discovery.

Key words

Cutaneous leishmaniasis (CL) Rodent models In vivo imaging IVIS Bioluminescence signal Luciferase-expressing Leishmania major Drug efficacy Base of the tail Footpad Ear infections 

Notes

Acknowledgments

Disclaimer: Material has been reviewed by the Walter Reed Army Institute of Research. There is no objection to its presentation and/or publication. The opinions or assertions contained herein are the private views of the author, and are not to be construed as official, or as reflecting true views of the Department of the Army or the Department of Defense.

Research was conducted under an approved animal use protocol in an AAALACi accredited facility in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, NRC Publication, 2011 edition.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Walter Reed Army Institute of ResearchSilver SpringUSA
  2. 2.Dwight D. Eisenhower Army Medical CenterFort GordonUSA

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