In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model

  • Shan Lei
  • Weijin Huang
  • Youchun Wang
  • Qiang LiuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2081)


Pseudoviruses are useful tools because of their safety and versatility compared to wild type viruses. Optical imaging of reporter gene labeled pseudoviruses in small animal models can allow for real-time analysis of the infection process without sacrificing the host, which has proven invaluable in the longitudinal study of disease events and testing the antiviral efficiencies of vaccine candidates, monoclonal antibodies and small molecule compounds. Here, we describe the generation of Marburg pseudovirus (pMARV) and establishment of imaging mouse model by using a deep-cooled CCD camera imager. We also describe the widespread organ distribution of pMARV during infection by ex vivo imaging of necropsied tissues. This system can significantly facilitate Marburg virus studies and enable the evaluation of treatments against MARV in BSL-2 containments.

Key words

Bioluminescence Surrogate disease model Marburg virus IVIS spectrum Alternative approach 



We are grateful to Ms. Yanqiu Li, Ms. Xiaoyue Shi, and Mr. Xing Huang from PerkinElmer Inc., for technical assistance. We also would like to thank Dr. Kevin Francis, PerkinElmer Inc. and Visiting Professor at UCLA and Texas A&M, and Mr. Joshua McHattan, PerkinElmer Inc. for language editing. This study was supported by grants from the National Natural Science Foundation of China (8187131465).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Shan Lei
    • 1
  • Weijin Huang
    • 1
  • Youchun Wang
    • 1
  • Qiang Liu
    • 1
    Email author
  1. 1.Division of HIV/AIDS and Sex-Transmitted Virus VaccinesNational Institutes for Food and Drug ControlBeijingChina

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