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Measuring MIF in Biological Fluids

  • Fabien B. Vincent
  • Tali LangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Abstract

MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.

Key words

MIF Serum Plasma Urine Cell culture ELISA Multiplex immunoassay Quantibody® 

References

  1. 1.
    Lang T et al (2015) MIF: implications in the pathoetiology of systemic lupus erythematosus. Front Immunol 6:577CrossRefGoogle Scholar
  2. 2.
    Bloom BR, Bennett B (1966) Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science 153(3731):80–82CrossRefGoogle Scholar
  3. 3.
    David JR (1966) Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci U S A 56(1):72–77CrossRefGoogle Scholar
  4. 4.
    Baugh JA, Bucala R (2002) Macrophage migration inhibitory factor. Crit Care Med 30(1 Supp):S27–S35CrossRefGoogle Scholar
  5. 5.
    Lan HY (2008) Role of macrophage migration inhibition factor in kidney disease. Nephron Exp Nephrol 109(3):e79–e83CrossRefGoogle Scholar
  6. 6.
    Calandra T, Roger T (2003) Macrophage migration inhibitory factor: a regulator of innate immunity. Nat Rev Immunol 3(10):791–800CrossRefGoogle Scholar
  7. 7.
    Vincent FB et al (2019) Serum and urinary macrophage migration inhibitory factor (MIF) in primary Sjogren’s syndrome. Joint Bone Spine 86(3):393–395CrossRefGoogle Scholar
  8. 8.
    Vincent FB et al (2018) Analysis of serum macrophage migration inhibitory factor and D-dopachrome tautomerase in systemic sclerosis. Clin Transl Immunol 7(12):e1042CrossRefGoogle Scholar
  9. 9.
    Vincent FB et al (2018) Analysis of urinary macrophage migration inhibitory factor in systemic lupus erythematosus. Lupus Sci Med 5(1):e000277CrossRefGoogle Scholar
  10. 10.
    Vincent FB et al (2019) Effect of storage duration on cytokine stability in human serum and plasma. Cytokine 113:453–457CrossRefGoogle Scholar
  11. 11.
    Tuck MK et al (2009) Standard operating procedures for serum and plasma collection: early detection research network consensus statement standard operating procedure integration working group. J Proteome Res 8(1):113–117CrossRefGoogle Scholar
  12. 12.
    Keustermans GC et al (2013) Cytokine assays: an assessment of the preparation and treatment of blood and tissue samples. Methods 61(1):10–17CrossRefGoogle Scholar
  13. 13.
    Thomas CE et al (2010) Urine collection and processing for protein biomarker discovery and quantification. Cancer Epidemiol Biomark Prev 19(4):953–959CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical CentreMonash UniversityClaytonAustralia
  2. 2.The Szalmuk Family Department of Medical Oncology, Level 2Cabrini InstituteMalvernAustralia

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