Measuring MIF in Biological Fluids

  • Fabien B. Vincent
  • Tali LangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)


MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.

Key words

MIF Serum Plasma Urine Cell culture ELISA Multiplex immunoassay Quantibody® 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical CentreMonash UniversityClaytonAustralia
  2. 2.The Szalmuk Family Department of Medical Oncology, Level 2Cabrini InstituteMalvernAustralia

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