Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo
In vivo visualization of the microvasculature of the mouse cremaster muscle has been fruitful in the evaluation of the role of macrophage migration inhibitory factor in promotion of leukocyte trafficking. Here we explain how to undertake this preparation, including details on mouse anesthesia, securing intravenous access, and cremaster muscle exteriorization. We also provide information on the various microscopy modalities now available for imaging microvascular preparations of this nature.
Key wordsIntravital microscopy Leukocyte interactions Rolling Adhesion Transmigration Cremaster muscle Postcapillary venule
This work is supported by funding from the National Health and Medical Research Council of Australia (1042775, MJH) and the Monash University School of Clinical Sciences at Monash Health (MUN).