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Purification of Antibodies Against Entamoeba histolytica MIF and Their Use in Analyzing Human and Mouse Samples

  • Laura Farr
  • Koji Watanabe
  • Shannon MoonahEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Abstract

Macrophage migration inhibitory factor (MIF) is a proinflammatory and proproliferative cytokine expressed in humans. MIF homologs also exist in many pathogenic protozoans, including Entamoeba, Plasmodium, Toxoplasma, and Leishmania. Production of antibodies against parasite proteins allows for the generation of assays to measure and visualize parasite infection within hosts. In this chapter, we describe how to specifically purify antibodies against Entamoeba histolytica MIF (EhMIF), and subsequently use anti-EhMIF antibodies for ELISA on mouse and human samples and for immunohistochemistry on human tissue. These methods can be applied to any protein for high-quality antibody purification.

Key words

Amebiasis Entamoeba Parasite 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.University of Virginia School of MedicineCharlottesvilleUSA
  2. 2.National Center for Global Health and MedicineShinjukuJapan
  3. 3.Department of MedicineUniversity of Virginia School of MedicineCharlottesvilleUSA

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