Chimeric RNA and Exosomes-Based Liquid Biopsy
Exosomes are considered as sources of disease biomarkers since they are stable carriers of genetic material and proteins. We recently demonstrated that chimeric RNA in exosomes derived from patient fluids can be detected and further used for disease diagnosis. Here we describe a systematic method to obtain exosomes from body fluids for detecting expression of specific chimeric RNA based on a RT-qPCR strategy.
Key wordsExosomal chimeric RNA Liquid biopsy Disease diagnosis Exosome extraction and purification RT-qPCR
This work was supported in part by National Natural Science Foundation of China (81572876, 81773087, 81071736, and 30973508 to H.Z.).