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Chimeric RNA pp 155-166 | Cite as

Overexpression of Chimeric RNA by Retroviral Transduction

  • Hao Wu
  • Hui LiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2079)

Abstract

Cloning the open reading frame (ORF) of a protein-coding chimeric RNA into a mammalian expression vector is a simple, practiced way to study the function of the chimeric RNA. Here, we provide procedures for the insertion of the RRM2-C2orf48 fusion ORF into a mammalian expression vector, achieving the overexpression of the RRM2-C2orf48 fusion protein in a colon cancer cell line by retroviral transduction.

Key words

Chimeric RNA Overexpression Plasmid Viral transduction 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Gastrointestinal SurgeryThe Third Xiangya Hospital of Central South UniversityChangshaChina
  2. 2.Department of Pathology, School of MedicineUniversity of VirginiaCharlottesvilleUSA
  3. 3.Department of Biochemistry and Molecular Genetics, School of MedicineUniversity of VirginiaCharlottesvilleUSA

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