Detection and Characterization of Transposons in Bacteria
Bacterial transposons, through their ability to transfer DNA sequences from one position in the genome to another, play a central role in the shape and the evolution of genomes. Extensive studies have been performed during the last five decades to understand the molecular mechanisms involved in the transposition of a variety of elements. Among the methods used, the papillation and the mating out coupled to arbitrary primed PCR assays described in this chapter are widely used as very powerful approaches to detect and characterize transposition events in vivo.
Key wordsTransposition Transposon Mating out Arbitrary primed PCR Papillation
We thank Catherine Turlan for careful reading and suggestions and François Cornet, Jean-Yves Bouet, and Roxanne Diaz for discussions concerning this manuscript. This work was supported by the Agence National pour la Recherche (ANR-12-BSV8-0009-01).
- 3.Siguier P, Gourbeyre E, Varani A, Ton-Hoang B, Chandler M (2015) Everyman’s guide to bacterial insertion sequences. Microbiol Spectr 3(2). https://doi.org/10.1128/microbiolspec.MDNA3-0030-2014. MDNA3-0030-2014. Review. PMID: 26104715
- 22.Pasternak C, Ton-Hoang B, Coste G, Bailone A, Chandler M, Sommer S (2010) Irradiation-induced Deinococcus radiodurans genome fragmentation triggers transposition of a single resident insertion sequence. PLoS Genet 6(1):e1000799. https://doi.org/10.1371/journal.pgen.1000799CrossRefPubMedPubMedCentralGoogle Scholar