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Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health

  • Ana R. FreitasEmail author
  • Carla Novais
  • Luísa Peixe
  • Teresa M. CoqueEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2075)

Abstract

This chapter describes the methods to extract and characterize plasmids of Gram-positive bacterial species of interest in public health (biomedicine, veterinary, and food safety) as Staphylococcus, Streptococcus, Enterococcus, Listeria, and Clostridium and lactic acid bacteria. References for detailed plasmid classification are given in order to provide a comprehensive landscape in the interpretation of their plasmidomes.

Key words

Plasmids Plasmidome DNA extraction Restriction digest S1 nuclease Pulsed-field gel electrophoresis Southern transfer Hybridization 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Laboratory of Microbiology, Biological Sciences Department, Faculty of Pharmacy, UCIBIO/REQUIMTEUniversity of PortoPortoPortugal
  2. 2.Department of MicrobiologyRamón y Cajal Research Institute (IRYCIS)MadridSpain
  3. 3.CIBER in Epidemiology and Public Health (CIBERESP)MadridSpain

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