Peroxiredoxin Proteins as Building Blocks for Nanotechnology

  • Frankie Conroy
  • N. Amy YewdallEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2073)


Peroxiredoxins are ubiquitous antioxidant proteins that exhibit a striking variety of quaternary structures, making them appealing building blocks with which nanoscale architectures are created for applications in nanotechnology. The solution environment of the protein, as well as protein sequence, influences the presentation of a particular structure, thereby enabling mesoscopic manipulations that affect arrangments at the nanoscale. This chapter will equip us with the knowledge necessary to not only produce and manipulate peroxiredoxin proteins into desired structures but also to characterize the different structures using dynamic light scattering, analytical centrifugation, and negative stain transmission electron microscopy, thereby setting the stage for us to use these proteins for applications in nanotechnology.

Key words

Protein Nanotechnology Peroxiredoxin Prx3 Protein engineering Self-assembly Dynamic light scattering Analytical ultracentrifugation Transmission electron microscopy Negative stain 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.School of Biological SciencesUniversity of AucklandAucklandNew Zealand
  2. 2.Bio-Organic ChemistryEindhoven University of TechnologyEindhovenThe Netherlands

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