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Protein Expression in the Baculovirus-Insect Cell Expression System

  • Adetayo A. Adeniyi
  • Linda Hwee-Lin LuaEmail author
Protocol
  • 751 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2073)

Abstract

The baculovirus-insect cell expression system is a popular choice for recombinant protein production. Post-translational modifications, production of protein complexes, and reported high protein yields are some of the favorable features of this eukaryotic expression system. The intricacies of the baculovirus-insect cell expression system may deter beginners from implementing it for routine protein production. This chapter documents a detailed protocol for using the baculovirus-insect cell expression system, which has been verified in a protein production research facility.

Key words

Baculovirus Insect cell Protein expression Bacmid-mediated Homologous recombination-mediated Spodoptera frugiperda Trichoplusia ni 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.UQ Protein Expression FacilityThe University of QueenslandBrisbaneAustralia

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