Protein Expression in the Baculovirus-Insect Cell Expression System
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The baculovirus-insect cell expression system is a popular choice for recombinant protein production. Post-translational modifications, production of protein complexes, and reported high protein yields are some of the favorable features of this eukaryotic expression system. The intricacies of the baculovirus-insect cell expression system may deter beginners from implementing it for routine protein production. This chapter documents a detailed protocol for using the baculovirus-insect cell expression system, which has been verified in a protein production research facility.
Key wordsBaculovirus Insect cell Protein expression Bacmid-mediated Homologous recombination-mediated Spodoptera frugiperda Trichoplusia ni
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