Atomic Force Microscopy of Proteins

  • Yiran An
  • Sesha Sarathchandra Manuguri
  • Jenny MalmströmEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2073)


Atomic force microscopy (AFM) enables imaging of surface-deposited proteins and protein structures under physiological conditions, which is a benefit compared to ultra-high vacuum techniques such as electron microscopy. AFM also has the potential to provide more information from the phase in tapping mode or from functional AFM modes. The sample preparation, probe selection, and imaging conditions are crucial for successful imaging of proteins. Here we give a detailed account of the steps toward imaging of soft samples in both air and liquid along with the basic theory underpinning these details.

Key words

Atomic force microscopy Proteins Sample preparation Probe selection Imaging conditions 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Yiran An
    • 1
    • 2
  • Sesha Sarathchandra Manuguri
    • 1
    • 2
    • 3
  • Jenny Malmström
    • 2
    • 3
    Email author
  1. 1.School of Chemical SciencesUniversity of AucklandAucklandNew Zealand
  2. 2.Department of Chemical and Materials EngineeringUniversity of AucklandAucklandNew Zealand
  3. 3.MacDiarmid Institute for Advanced Materials and NanotechnologyAucklandNew Zealand

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