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A Low-Cost High-Throughput Method for Plant Genomic DNA Isolation

  • Prateek Gupta
  • Hymavathi Salava
  • Yellamaraju Sreelakshmi
  • Rameshwar Sharma
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2072)

Abstract

Many of the functional genomics methods require isolation of genomic DNA from large population of plants. The selection of DNA isolation protocols depends on several factors such as choice of starting material, ease of handling, time and labor required for isolation, the final quantity as well as the quality of genomic DNA. We outline here a high-throughput method of DNA extraction from different plant species including cereal crops. The protocol can be used for extraction of DNA in single tubes as well as for large formats in 96-well plates. The protocol includes steps for eliminating interfering secondary products such as phenolics. This protocol can be applied for high-throughput isolation of DNA for varied applications such as TILLING, mapping, fingerprinting, etc. as a cost-effective protocol compared to commercial kits.

Key words

DNA isolation Genomic DNA Corn Maize Cereals 

Notes

Acknowledgments

The development of this protocol was supported by the Department of Biotechnology, New Delhi, India.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Prateek Gupta
    • 1
  • Hymavathi Salava
    • 1
  • Yellamaraju Sreelakshmi
    • 1
  • Rameshwar Sharma
    • 1
  1. 1.Repository of Tomato Genomics Resources, Department of Plant SciencesUniversity of HyderabadHyderabadIndia

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