Sub-culturing of Bacteria, Fungi and Actinomycetes

  • Aakanchha Jain
  • Richa Jain
  • Sourabh Jain
Part of the Springer Protocols Handbooks book series (SPH)


Microbial growth is defined as increase in number and/or biomass. All microorganisms require food, oxygen, moisture, and space for growth. Like any other living organisms, they age, and their growth is inhibited in the absence of food, space, oxygen, etc. In order to sustain any microbial culture in laboratory conditions, sub-culturing is required. Sub-culturing is a procedure of transferring of microorganism into fresh nutritive medium from its stock culture. It includes transfer of culture from slant to slant, slant to plate, plate to plate, plate to slant, solid medium to broth, and broth to solid media. Sub-culturing is done to maintain culture in its active form (prolonging life and/or increase the number of cells) for varied applications.

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Aakanchha Jain
    • 1
  • Richa Jain
    • 2
  • Sourabh Jain
    • 3
  1. 1.Bhagyoday Tirth Pharmacy CollegeSagarIndia
  2. 2.Centre for Scientific Research and DevelopmentPeople’s UniversityBhopalIndia
  3. 3.Sagar Institute of Pharmaceutical SciencesSagarIndia

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