Estimation of DNA by Diphenylamine Reaction

  • Aakanchha Jain
  • Richa Jain
  • Sourabh Jain
Part of the Springer Protocols Handbooks book series (SPH)


In the presence of acid, the 2-deoxypentose of DNA is converted to beta-hydroxylevulinaldehyde which reacts with diphenylamine to give blue color, which is estimated by UV spectrophotometer or by colorimeter at 595 nm. Since only deoxyribose of purine nucleotide reacts, the value obtained represents only half to total amount.

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Aakanchha Jain
    • 1
  • Richa Jain
    • 2
  • Sourabh Jain
    • 3
  1. 1.Bhagyoday Tirth Pharmacy CollegeSagarIndia
  2. 2.Centre for Scientific Research and DevelopmentPeople’s UniversityBhopalIndia
  3. 3.Sagar Institute of Pharmaceutical SciencesSagarIndia

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