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Extraction of DNA and RNA from Microorganism

  • Aakanchha Jain
  • Richa Jain
  • Sourabh Jain
Protocol
  • 75 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Principle: The enzyme lysozyme is used for the rupturing of cell wall of bacteria or microorganism. The high salt concentration prevents the conversion of dsDNA to ssDNA. EDTA and sodium citrate present helps to chelate metal ions which activate DNase and RNase enzymes. The protein denaturation is done by sodium chlorate and finally extraction is done either by ethanol or isopropanol method as discussed earlier in section.

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Aakanchha Jain
    • 1
  • Richa Jain
    • 2
  • Sourabh Jain
    • 3
  1. 1.Bhagyoday Tirth Pharmacy CollegeSagarIndia
  2. 2.Centre for Scientific Research and DevelopmentPeople’s UniversityBhopalIndia
  3. 3.Sagar Institute of Pharmaceutical SciencesSagarIndia

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