Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR

  • Engil I. P. Pereira
  • Marcelo C. M. Teixeira FilhoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2057)


Nitrification is the microbial-mediated transformation of ammonium (NH4+) into nitrate (NO3). Many plant species depend on the availability of NO3 as the main source of nitrogen (N). On the other hand, because NO3 is highly mobile in the soil profile, its excess concentration can cause environmental pollution. Nitrification can be estimated at the process level, but with the development of molecular techniques it is also possible to estimate the abundance of nitrifying bacteria in the soil. Hence, in this chapter we describe the procedure for detection and quantification of nitrifying bacteria in soil samples using real-time quantitative polymerase chain reaction (PCR).

Key words

Nitrification Nitrosomonas europaea Nitrifying gene DNA extraction Amplification efficiency 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Engil I. P. Pereira
    • 1
  • Marcelo C. M. Teixeira Filho
    • 2
    Email author
  1. 1.School of Earth, Environmental, and Marine SciencesThe University of Texas Rio Grande ValleyEdinburgUSA
  2. 2.Department of Plant Protection, Rural Engineering and SoilsSão Paulo State University (UNESP)Ilha SolteiraBrazil

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