Functional Genome Profiling to Understand Cancer Immune Responsiveness

  • Ena Wang
  • Davide Bedognetti
  • Francesco M. Marincola
Part of the Methods in Molecular Biology book series (MIMB, volume 2055)


It has been almost two decades since we first proposed the use of minimally invasive serial biopsies to dissect the biology underlining cancer immune responsiveness (CIR) by looking for predictors of response, understanding mechanisms of action (MOA) of therapeutics and documenting strategies adopted by tumor cells to escape immune recognition. This approach led to the first description in 2002 of predictors of CIR, the characterization of the pharmacodynamics of several immune therapeutics, and the geneses of immune escape under immunological pressure prompted by successful treatment. The presumption was straightforward; study CIR where it occurs: the target organ. Since then, a large number of studies corroborated these early observations adding sophistication and accuracy to the investigations. Here, we summarize the history of functional genomic profiling as a discovery and validation tool for immune oncology (IO) and new insights that could be derived by single novel technologies.

Key words

Genomic profiling Immune oncology (IO) Cancer immune responsiveness (CIR) Mechanisms of action (MOA) 



Adoptive cellular therapy


C-C motif chemokine receptor


Cancer immune responsiveness


Damage associated molecular pattern


High-mobility group box protein 1


Immunogenic cell death


Immunologic constant of rejection


Immune-checkpoint inhibitor therapy




Immune oncology


Major histocompatibility complex


Mechanism of action


The Cancer Genome Atlas


Tumor-infiltrating lymphocytes


Tumor inflammation signature


Tumor microenvironment


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Ena Wang
    • 1
  • Davide Bedognetti
    • 2
  • Francesco M. Marincola
    • 3
  1. 1.Allogene TherapeuticsSouth San FranciscoUSA
  2. 2.Sidra MedicineDohaQatar
  3. 3.Refuge BiotechnologiesMenlo ParkUSA

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