Preparation of Cryptosporidium DNA for Whole Genome Sequencing
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The application of whole genome sequencing (WGS) to Cryptosporidium has been challenged by the relatively small numbers of oocysts present in feces and lack of suitable culture systems to generate sufficient DNA that is required. Here, we provide a method for the preparation of sufficient, high-quality Cryptosporidium DNA for WGS from feces, incorporating parasite purification stages and an optional whole genome amplification step.
KeywordsCryptosporidium DNA extraction Whole genome sequencing WGS Whole genome amplification
The research leading to these methods has received funding from the European Union Seventh Framework Programme ([FP7/2007-2013] [FP7/2007-2011]) under grant agreement no. 311846.
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