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Preparation of Cryptosporidium DNA for Whole Genome Sequencing

  • Guy Robinson
  • Rachel M. ChalmersEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2052)

Abstract

The application of whole genome sequencing (WGS) to Cryptosporidium has been challenged by the relatively small numbers of oocysts present in feces and lack of suitable culture systems to generate sufficient DNA that is required. Here, we provide a method for the preparation of sufficient, high-quality Cryptosporidium DNA for WGS from feces, incorporating parasite purification stages and an optional whole genome amplification step.

Keywords

Cryptosporidium DNA extraction Whole genome sequencing WGS Whole genome amplification 

Notes

Acknowledgments

The research leading to these methods has received funding from the European Union Seventh Framework Programme ([FP7/2007-2013] [FP7/2007-2011]) under grant agreement no. 311846.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Cryptosporidium Reference Unit, Public Health Wales Microbiology and Health ProtectionSingleton HospitalSwanseaUK

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