Cryptosporidium Diagnostic Assays: Molecular Detection
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Molecular diagnostic assays for Cryptosporidium are usually based on PCR and may detect the entire genus or target specified species. Of the ~40 species, fewer than half have been reported from humans, and most human cases of cryptosporidiosis are caused by Cryptosporidium parvum or Cryptosporidium hominis. Here we describe a nested PCR for the detection of all Cryptosporidium spp. that can then be differentiated by sequencing the PCR amplicons, and a duplex, real-time PCR for the simultaneous detection and differentiation of C. parvum and C. hominis.
KeywordsCryptosporidium C. parvum C. hominis Detection DNA extraction Nested PCR Real-time PCR
The research leading to the development of the duplex real-time PCR described here has received funding from the European Union Seventh Framework Program [(FP7/2007-2013) (FP7/2007-2011)] under Grant agreement no: 311846.
- 1.Smith H (2008) Diagnostics. In: Fayer R, Xiao L (eds) Cryptosporidium and cryptosporidiosis, 2nd edn. CRC Press, Boca RatonGoogle Scholar
- 10.Moore CE, Elwin K, Phot N, Seng C, Mao S, Suy K, Kumar V, Nader J, Bousfield R, Perera S, Bailey JW, Beeching NJ, Day NP, Parry CM, Chalmers RM (2016) Molecular characterisation of Cryptosporidium species and Giardia duodenalis from symptomatic Cambodian children. PLoS Negl Trop Dis 10:e0004822CrossRefGoogle Scholar