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Cryptosporidium Diagnostic Assays: Molecular Detection

  • Guy Robinson
  • Kristin Elwin
  • Rachel M. ChalmersEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2052)

Abstract

Molecular diagnostic assays for Cryptosporidium are usually based on PCR and may detect the entire genus or target specified species. Of the ~40 species, fewer than half have been reported from humans, and most human cases of cryptosporidiosis are caused by Cryptosporidium parvum or Cryptosporidium hominis. Here we describe a nested PCR for the detection of all Cryptosporidium spp. that can then be differentiated by sequencing the PCR amplicons, and a duplex, real-time PCR for the simultaneous detection and differentiation of C. parvum and C. hominis.

Keywords

Cryptosporidium C. parvum C. hominis Detection DNA extraction Nested PCR Real-time PCR 

Notes

Acknowledgments

The research leading to the development of the duplex real-time PCR described here has received funding from the European Union Seventh Framework Program [(FP7/2007-2013) (FP7/2007-2011)] under Grant agreement no: 311846.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Guy Robinson
    • 1
  • Kristin Elwin
    • 1
  • Rachel M. Chalmers
    • 1
    Email author
  1. 1.Cryptosporidium Reference Unit, Public Health Wales Microbiology and Health ProtectionSingleton HospitalSwanseaUK

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