High-Content Screening for Cryptosporidium Drug Discovery

  • Melissa S. Love
  • Case W. McNamaraEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2052)


High-content screening (HCS) is a cell-based type of phenotypic screening that combines multiple simultaneous readouts with a high level of throughput. A particular benefit of this form of screening for drug discovery is the ability to perform the interrogation in a biologically relevant system. This approach has greatly advanced the field of drug discovery for cryptosporidiosis, a diarrheal disease caused by protozoan parasites of Cryptosporidium spp. These parasites are obligate intracellular parasites and cannot be cultured in vitro without the support of a host cell, limiting the options for potential assay readout. Here we describe an established 384- or 1536-well format high-content imaging (HCI) assay of Cryptosporidium-infected HCT-8 human ileocecal adenocarcinoma cells. This HCS assay is a powerful tool to assess large numbers of compounds to power drug discovery, as well as to phenotypically characterize known Cryptosporidium-active compounds.


Phenotypic screening High-throughput screening High-content imaging Cryptosporidium Cryptosporidiosis Anti-cryptosporidial Drug discovery Neglected tropical diseases Infectious diseases 


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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Calibr at Scripps ResearchLa JollaUSA

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