Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water

  • Jennifer L. MurphyEmail author
  • Michael J. Arrowood
Part of the Methods in Molecular Biology book series (MIMB, volume 2052)


This chapter provides a detailed protocol to assess disinfection efficacy of chlorine against Cryptosporidium oocysts including the core chlorine disinfection assay, the in vitro cell culture infectivity assay, and microscopy analysis and data interpretation.


Cell culture infectivity Chlorine Disinfection In vitro culture Cryptosporidium Oocysts 



The authors would like to acknowledge the expert assistance of Long Ti Xie, who helped to optimize the culture methods and parasite quantification in the immunofluorescence assays.

Disclosures: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Division of Foodborne, Waterborne, and Environmental DiseasesNational Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and PreventionAtlantaUSA

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