Low-Voltage Flow-Through Electroporation Membrane and Method
Electroporation uses high electric field gradients to create pores within the membrane of living cells in order to deliver a substance, for example a gene, into the cytoplasm. To achieve such gradients, current electroporation devices deliver voltage pulses in the kV range to the cell medium. We describe here a new device based on gold-microtube membranes that can accomplish electroporation with voltage pulses that are orders of magnitude smaller, 4 V. The percentages of electroporated bacteria were found to be more than an order of magnitude higher than obtained with a commercial electroporator.
Key wordsElectroporation Gold-microtube membranes Escherichia coli Electric field gradient
This work was supported by the University of Florida.
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