Ultrahigh-Density Screens for Genome-Wide Yeast EMAPs in a Single Plate
Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.
Key wordsEMAP Genetic interactions Synthetic lethality Ultrahigh-density yeast arrays S-scores
This work was supported by NIH grants L30 CA171000 to J.P.S. and R01 GM084279, R01 ES014811, and U54 CA209891 to T.I.
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