Probing the RNA-Binding Proteome from Yeast to Man: Major Advances and Challenges

  • Benedikt M. BeckmannEmail author
  • Sander Granneman
Part of the Methods in Molecular Biology book series (MIMB, volume 2049)


RNA-binding proteins are important for core cellular processes such as mRNA transcription, splicing, transport, translation, and degradation. Recently, hundreds of novel RNA-binders have been identified in vivo in various organisms and cell types. We discuss the RNA interactome capture technique which enabled this boost in identifying new RNA-binding proteins in eukaryotes. A focus of this chapter, however, is the presentation of different challenges and problems that need to be addressed to be able to understand the conserved mRNA-bound proteomes from yeast to man.

Key words

RNA-binding proteins mRNA processing Proteome Eukaryotes 



B.M.B. wishes to thank Matthias Hentze for discussions and comments on the chapter.


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Authors and Affiliations

  1. 1.Molecular Infection Biology, IRI Life SciencesHumboldt-Universität zu BerlinBerlinGermany
  2. 2.Centre for Systems and Synthetic Biology (SynthSys)University of EdinburghEdinburghUK

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