Data-Independent Acquisition for Yeast Glycoproteomics
Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins. Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure. Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis of structure and occupancy of N-glycans from yeast cell wall glycoproteins.
Key wordsGlycosylation Glycoprotein Mass spectrometry Data independent acquisition DIA SWATH Occupancy Macroheterogeneity Structure Microheterogeneity Yeast Cell wall
Funding: This work was supported by Australian Research Council Discovery Project grant DP160102766 and Australian Research Council Industrial Transformation and Training Centre Grant IC160100027 to BLS. BLS holds an Australian National Health and Medical Research Council RD Wright Biomedical Fellowship APP1087975.
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