Differentiating CD8αβ T Cells from TCR-Transduced iPSCs for Cancer Immunotherapy

  • Atsutaka Minagawa
  • Shin KanekoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2048)


The use of induced pluripotent stem cells (iPSCs) as a cell source for producing cytotoxic T lymphocytes (CTLs) is expected to have advantages in the antigen specificity, rejuvenation profile, and reproducible number of CTLs. We have developed the way to differentiate CD8αβ T cells from TCR-transduced iPSCs (TCR-iPSCs). These T cells express monoclonal expression of the transduced TCR. Generating CD8αβ CTLs from TCR-iPSC could contribute to safe and effective allogeneic regenerative T cell immunotherapies.

Key words

TCR transduction iPSCs T cell differentiation 


  1. 1.
    Restifo NP, Dudley ME, Rosenberg SA (2012) Adoptive immunotherapy for cancer: harnessing the T cell response. Nat Rev Immunol 12(4):269–281PubMedPubMedCentralCrossRefGoogle Scholar
  2. 2.
    Minagawa A, Kaneko S (2014) Rise of iPSCs as a cell source for adoptive immunotherapy. Hum Cell 27(2):47–50PubMedCrossRefGoogle Scholar
  3. 3.
    Kitayama S et al (2016) Cellular adjuvant properties, direct cytotoxicity of re-differentiated Valpha24 invariant NKT-like cells from human induced pluripotent stem cells. Stem Cell Reports 6(2):213–227PubMedPubMedCentralCrossRefGoogle Scholar
  4. 4.
    Nishimura T et al (2013) Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation. Cell Stem Cell 12(1):114–126PubMedCrossRefGoogle Scholar
  5. 5.
    Sugita S et al (2016) Lack of T cell response to iPSC-derived retinal pigment epithelial cells from HLA homozygous donors. Stem Cell Reports 7(4):619–634PubMedPubMedCentralCrossRefGoogle Scholar
  6. 6.
    Hashizume O et al (2015) Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci Rep 5:10434PubMedPubMedCentralCrossRefGoogle Scholar
  7. 7.
    Abe A et al (2015) An enhancer of the IL-7 receptor alpha-chain locus controls IL-7 receptor expression and maintenance of peripheral T cells. J Immunol 195(7):3129–3138PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and ApplicationKyoto UniversityKyotoJapan

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