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Differentiating CD8αβ T Cells from TCR-Transduced iPSCs for Cancer Immunotherapy

  • Atsutaka Minagawa
  • Shin KanekoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2048)

Abstract

The use of induced pluripotent stem cells (iPSCs) as a cell source for producing cytotoxic T lymphocytes (CTLs) is expected to have advantages in the antigen specificity, rejuvenation profile, and reproducible number of CTLs. We have developed the way to differentiate CD8αβ T cells from TCR-transduced iPSCs (TCR-iPSCs). These T cells express monoclonal expression of the transduced TCR. Generating CD8αβ CTLs from TCR-iPSC could contribute to safe and effective allogeneic regenerative T cell immunotherapies.

Key words

TCR transduction iPSCs T cell differentiation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and ApplicationKyoto UniversityKyotoJapan

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