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SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity

  • Martin TaubertEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2046)

Abstract

Stable isotope probing combined with metaproteomics enables the detection and characterization of active key species in microbial populations under near-natural conditions, which greatly helps to understand the metabolic functions of complex microbial communities. This is achieved by providing growth substrates labeled with heavy isotopes such as 13C, which will be assimilated into microbial biomass. After subsequent extraction of proteins and proteolytic cleavage into peptides, the heavy isotope enrichment can be detected by high-resolution mass spectrometric analysis, and linked to the functional and taxonomic characterization of these biomarkers. Here we provide protocols for obtaining isotopically labeled proteins and for downstream SIP-metaproteomics analysis.

Key words

Stable isotope probing Metaproteomics Microbial ecology Metabolic labeling Mass spectrometry 

Notes

Acknowledgments

I thank Nico Jehmlich for providing helpful inputs and for reviewing the manuscript.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Biological Sciences, Institute of BiodiversityFriedrich Schiller University JenaJenaGermany

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