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DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea

  • Xiuran Yin
  • Ajinkya C. Kulkarni
  • Michael W. FriedrichEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2046)

Abstract

Methylotrophic methanogenic archaea are an integral part of the carbon cycle in various anaerobic environments. Different from methylotrophic bacteria, methylotrophic methanogens assimilate both, the methyl compound and dissolved inorganic carbon. Here, we present DNA- and RNA-stable isotope probing (SIP) methods involving an effective labeling strategy using 13C-labeled dissolved inorganic carbon (DIC) as carbon source along with methanol as dissimilatory substrate.

Key words

Methylotrophic methanogens Stable isotope probing (SIP) Labeling strategy Dissolved inorganic carbon Methanol 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Xiuran Yin
    • 1
    • 2
    • 3
  • Ajinkya C. Kulkarni
    • 1
    • 2
    • 3
  • Michael W. Friedrich
    • 1
    • 2
    Email author
  1. 1.Microbial Ecophysiology Group, Faculty of Biology/ChemistryUniversity of BremenBremenGermany
  2. 2.MARUM—Center for Marine Environmental SciencesUniversity of BremenBremenGermany
  3. 3.International Max Planck Research School for Marine MicrobiologyMax Planck Institute for Marine MicrobiologyBremenGermany

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