Advertisement

Experimental Nerve Plexus Root Avulsion and Spinal Cord Implantation

  • Thomas Carlstedt
  • Mårten Risling
Protocol
Part of the Neuromethods book series (NM, volume 149)

Abstract

The nerve plexus is a complicated formation originating from cervical or lumbosacral spinal nerve roots. It has recently attracted considerable experimental as well as clinical interest.

The nerve roots are the only site in the peripheral nervous system where efferent-motor and afferent-sensory nerve fibers are separated. This situation offers a multitude of possibilities for stringent experimental studies of the peripheral but also the central nervous system. A dorsal root lesion offers an uncompromised opportunity to assess after various manipulations spinal cord regeneration in a binary context. The clinical situation of plexus root avulsion from the spinal cord—usually after a trauma—is in fact a medullary lesion and can be considered in a cohort of patients as a proxy for a human spinal cord injury.

This chapter discusses the normal anatomy and describes surgical approaches as well as lesions and repair of the nerve plexus experimentally.

Key words

Nerve plexus Root avulsion Laminectomy Spinal cord Replantation Tissue glue 

References

  1. 1.
    Jamieson AM, Eames RA (1980) Reimplantation of avulsed brachial plexus roots: an experimental study in dogs. Int J Microsurg 2:75–85Google Scholar
  2. 2.
    Carlstedt T, Lindå H, Cullheim S et al (1986) Reinnervation of hind limb muscles after ventral root avulsion and implantation in the lumbar spinal cord of the adult rat. Acta Physiol Scand 128:645–646CrossRefGoogle Scholar
  3. 3.
    Cullheim S, Carlstedt T, Lindå H, Risling M et al (1989) Motoneurons reinnervate skeletal muscle after ventral root implantation into the spinal cord. Neuroscience 29:725–733CrossRefGoogle Scholar
  4. 4.
    Carlstedt T (2007) Central nerve plexus injury. Imperial College Press, LondonCrossRefGoogle Scholar
  5. 5.
    Hendriks WTJ, Eggers R,Carlstedt TP, Zaldumbide A. et al. (2007) Lentiviral vector-mediated report gene expression in avulsed spinal ventral rroot is short-termed,but prolonged using an immune “stealth” transgene. Restor Neurol Neurosci 25:585–599Google Scholar
  6. 6.
    Hoffman CFE, Thomeer RTWM, Marani E (1993) Reimplantation of ventral rootlets into the cervical spinal cord after their avulsion: an anterior surgical approach. Clin Neurol Neurosurg 95(Suppl):112–118CrossRefGoogle Scholar
  7. 7.
    Li Y, Carlstedt T, Berthold C-H et al (2004) Interaction of transplanted olfactory-ensheathing host astrocytic processes provides a bridge for axons to regenerate across the dorsal root entry zone. Exp Neurol 188:300–308CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Thomas Carlstedt
    • 1
  • Mårten Risling
    • 2
  1. 1.The Wolfson Centre for Age-Related DiseasesKing’s College LondonLondonUK
  2. 2.Experimental Traumatology, Department of NeuroscienceKarolinska InstitutetStockholmSweden

Personalised recommendations