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Application of an Aptamer-Based Proteomics Assay (SOMAscan™) in Rat Cerebrospinal Fluid

  • Alba Simats
  • Laura Ramiro
  • Joan Montaner
  • Teresa García-Berrocoso
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2044)

Abstract

The exploration of the cerebrospinal fluid (CSF) through proteomics techniques might help in the search of molecular biomarkers relevant to neurological pathologies. Aiming this, we describe here a commercially available multiplexed proteomics technology based on the use of modified aptamers (SOMAscan™ assay). From our experience in exploring the rat CSF proteome, we detail the basic principles of this oligonucleotide-based proteomics approach, as well as the main data-processing steps to obtain relative quantitative values for proteins that could discriminate among different brain conditions, as an attempt in the search of neurological biomarkers. Finally, we give some tips on performing the SOMAscan assay and key recommendations on the verification analyses of the resulting candidate biomarkers.

Key words

Aptamer CSF Multiplex Proteome Rat SOMAscan™ 

Notes

Acknowledgement

This work was supported by a PI15/00354 grant from Fondo de Investigaciones Sanitarias of Instituto de Salud Carlos III (co-financed by the European Regional Development Fund, FEDER). Neurovascular Research Laboratory takes part into the Spanish stroke research network INVICTUS+ (RD16/0019). L. R is supported by a pre-doctoral fellowship from the Instituto de Salud Carlos III (IFI17/00012).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Alba Simats
    • 1
  • Laura Ramiro
    • 1
  • Joan Montaner
    • 1
  • Teresa García-Berrocoso
    • 1
  1. 1.Neurovascular Research Laboratory, Vall d’Hebron Institute of Research (VHIR)Universitat Autònoma de BarcelonaBarcelonaSpain

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