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Studying a Light Sensor with Light: Multiphoton Imaging in the Retina

  • Thomas EulerEmail author
  • Katrin Franke
  • Tom Baden
Protocol
Part of the Neuromethods book series (NM, volume 148)

Abstract

Two-photon imaging of light stimulus-evoked neuronal activity has been used to study all neuron classes in the vertebrate retina, from the photoreceptors to the retinal ganglion cells. Clearly, the ability to study retinal circuits down to the level of single synapses or zoomed out at the level of complete populations of neurons has been a major asset in our understanding of this beautiful circuit. In this chapter, we discuss the possibilities and pitfalls of using an all-optical approach in this highly light-sensitive part of the brain.

Keywords

Vertebrate retina Mouse Zebrafish Two-photon microscopy Biosensor Activity probes Visual stimulus-evoked activity Laser-evoked retinal activity 

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Authors and Affiliations

  1. 1.Institute for Ophthalmic ResearchUniversity of TübingenTübingenGermany
  2. 2.Werner Reichardt Centre for Integrative Neuroscience (CIN)University of TübingenTübingenGermany
  3. 3.Bernstein Centre for Computational Neuroscience TübingenUniversity of TübingenTübingenGermany
  4. 4.School of Life SciencesUniversity of SussexBrightonUK

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