Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA)

  • Kevin E. Eboigbodin
  • Mark Hoser
Part of the Methods in Molecular Biology book series (MIMB, volume 2042)


Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the most prevalent causes of sexually transmitted infections (STIs) worldwide. Timely and accurate diagnosis plays an important role in deciding appropriate treatment and preventing the spread of the infection. Strand invasion based amplification (SIBA), is an established isothermal nucleic acid amplification method for the rapid and accurate detection of infectious diseases. SIBA was applied for the simultaneous detection of CT and NG in less than 1 h. The multiplex SIBA (mSIBA) method displayed high analytical sensitivity and specificity for the detection of CT and NG. Since the method is performed at low and constant temperature, it can therefore be run on portable instruments. SIBA enables rapid screening for CT and NG within point-of-care or central laboratory settings.

Key words

Detection Diagnosis Strand invasion based amplification Multiplex Polymerase chain Chlamydia trachomatis Neisseria gonorrhoeae 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kevin E. Eboigbodin
    • 1
  • Mark Hoser
    • 2
  1. 1.Research and DevelopmentOrion Diagnostica OyEspooFinland
  2. 2.Molecular BiologyGeneForm TechnologiesBroadstairsUK

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