Stable Isotope-Resolved Metabolomics by NMR

  • Penghui Lin
  • Andrew N. LaneEmail author
  • Teresa W.-M. Fan
Part of the Methods in Molecular Biology book series (MIMB, volume 2037)


Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.

Key words

Stable isotope-resolved metabolomics Isotopomers Spectral editing Quantification 





Free induction decay




Region of interest


Stable isotope-resolved metabolomics


Signal-to-noise ratio





This work was supported in part by NIH grants 1U24DK097215-01A1, CA163223-01A1, 5P20GM121327 (COBRE) the Carmen L. Buck endowment (to ANL), and the Edith D. Gardner Chair in Cancer Research (TWMF).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Penghui Lin
    • 1
  • Andrew N. Lane
    • 1
    • 2
    Email author
  • Teresa W.-M. Fan
    • 1
    • 2
  1. 1.Center for Environmental and Systems BiochemistryUniversity of KentuckyLexingtonUSA
  2. 2.Department of Toxicology and Cancer BiologyUniversity of KentuckyLexingtonUSA

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